Primer

Artistry and super-heroics collide ain this new superhero story!

Author: Jennifer Muro

Publisher: National Geographic Books

ISBN: 9781401296575

Category: Juvenile Fiction

Page: 0

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Artistry and super-heroics collide ain this new superhero story! Thirteen-year-old Ashley Rayburn is an upbeat girl with a decidedly downbeat past. With a father in prison, Ashley has bounced from foster home to foster home and represents a real challenge to the social workers who try to help her--not because she's inherently bad, but because trouble always seems to find her. Things start looking up for Ashley when she finds new, loving parents, a best friend, and an outlet for her creative skills. But her life quickly gets more complicated when she also finds a suitcase full of specially enhanced body paints, changing her from artist to the world's newest superhero. It's the greatest thing to happen in her life so far, until Ashley finds herself pursued by a government agency that wants those paints back! Now she has to make hard choices to protect her new parents and learn what it truly means to be a family. From writers Jennifer Muro (Star Wars: Forces of Destiny) and Thomas Krajewski (Netflix's Buddy Thunderstruck) and artist Gretel Lusky comes a brand-new superhero for the DC Universe in a story that is alternately fun, dramatic, emotional, and uplifting.

Emerging Approaches for Typing Detection Characterization and Traceback of Escherichia coli 2nd Edition

All primer pairs were targeted unique sequences of wzy, except for OgN31 for which primers were targeted to a glycosyltransferase gene. Each PCR was evaluated by using all 185 O-serogroup reference strains from O1 to O188 and six novel ...

Author: Pina M. Fratamico

Publisher: Frontiers Media SA

ISBN: 9782889454334

Category:

Page: 172

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Pathogenic Escherichia coli strains cause a large number of diseases in humans, including diarrhea, hemorrhagic colitis, hemolytic uremic syndrome, urinary tract infections, and neonatal meningitis, while in animals they cause diseases such as calf scours and mastitis in cattle, post-weaning diarrhea and edema disease in pigs, and peritonitis and airsacculitis in chickens. The different E. coli pathotypes are characterized by the presence of specific sets of virulence-related genes. Therefore, it is not surprising that pathogenic E. coli constitutes a genetically heterogeneous family of bacteria, and they are continuing to evolve. Rapid and accurate molecular methods are critically needed to detect and trace pathogenic E. coli in food and animals. They are also needed for epidemiological investigations to enhance food safety, as well as animal and human health and to minimize the size and geographical extent of outbreaks. The serotype of E. coli strains has traditionally been determined using antisera raised against the >180 different O- (somatic) and 53 H- (flagellar) antigens. However, there are many problems associated with serotyping, including: it is labor-intensive and time consuming; cross reactivity of the antisera with different serogroups occurs; antisera are available only in specialized laboratories; and many strains are non-typeable. Molecular serotyping targeting O-group-specific genes within the E. coli O-antigen gene clusters and genes that are involved in encoding for the different flagellar types offers an improved approach for determining the E. coliO- and H-groups. Furthermore, molecular serotyping can be coupled with determination of specific sets of virulence genes carried by the strain offering the possibility to determine O-group, pathotype, and the pathogenic potential simultaneously. Sequencing of the O-antigen gene clusters of all of the known O-groups of E. coli is now complete, and the sequences have been deposited in the GenBank database. The sequence information has revealed that some E. coli serogroups have identical sequences while others have point mutations or insertion sequences and type as different serogroups in serological reactions. There are also a number of other ambiguities in serotyping that need to be resolved. Furthermore, new E. coli O-groups are being identified. Therefore, there is an essential need to resolve these issues and to revise the E. coli serotype nomenclature based on these findings. There are emerging technologies that can potentially be applied for molecular serotyping and detection and characterization of E. coli. On a related topic, the genome sequence of thousands of E. coli strains have been deposited in GenBank, and this information is revealing unique markers such as CRISPR (clustered regularly interspaced short palindromic repeats) and virulence gene markers that could be used to identify E. coli pathotypes. Whole genome sequencing now provides the opportunity to study the role of horizontal gene transfer in the evolution and emergence of pathogenic E. coli strains. Whole genome sequencing approaches are being investigated for genotyping and outbreak investigation for regulatory and public health needs; however, there is a need for establishing bioinformatics pipelines able to handle large amounts of data as we move toward the use of genetic approaches for non-culture-based detection and characterization of E. coli and for outbreak investigations.

A Primer of the Novel

In this revised edition, David Madden, Charles Bane and Sean Flory have produced an updated work that is intended for a general readership including writers, teachers, and students who are just being introduced to the genre.

Author: David Madden

Publisher:

ISBN: STANFORD:36105114416527

Category: Literary Criticism

Page: 278

View: 797

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In this revised edition, David Madden, Charles Bane and Sean Flory have produced an updated work that is intended for a general readership including writers, teachers, and students who are just being introduced to the genre.

The Dialogic Novels of Malcolm Bradbury and David Lodge

There is no dominant key and no dominant language either , only languages and minimalist variations in this , the most artful and the most artificial of Bradbury's four novels . The " primer type of narration " identified as such a key ...

Author: Robert A. Morace

Publisher: SIU Press

ISBN: 080931519X

Category: Literary Criticism

Page: 252

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Discusses the overlooked works of Bradbury and Lodge in terms of their critical reception, Bakhtin's theory of the dialogical novel, and their relation to British literature and contemporary literature in general. Annotation copyright Book News, Inc. Portland, Or.

Toni Morrison s Secret Drive

the words—and by the association of a reading primer with innocent children. As the text later reveals, the novel is, in fact, about unspeakably horrible things happening to a child. Although the final version of the primer extract ...

Author: David S. Goldstein

Publisher: McFarland

ISBN: 9781476641096

Category: Literary Criticism

Page: 200

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The late Toni Morrison was the first African-American to win the Nobel Prize for Literature in 1993. A powerful writer, she wove stories depicting the largely overlooked Black experience in America and exploring the intersection of gender and race through the lives of Black women. Morrison's writing continues to move people and push readers to reassess their beliefs about what it means to be Black in America. Synthesizing some 250 scholarly works about Morrison's writing, this book examines eight novels as well as the short story "Recitatif." They are analyzed for techniques used to deepen meaning and emotional weight, and reveal Morrison's mastery over prose.

Scenes of Shame

( 3 ) Morrison explains that she uses the Dick and Jane primer story , with its depiction of a happy white family ... Thus the chapters of the novel that are headed with the primer descriptions of Jane's idealized " green and white ...

Author: Joseph Adamson

Publisher: SUNY Press

ISBN: 0791439763

Category: Psychology

Page: 300

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Explores the role of shame as an important affect in the complex psychodynamics of literary and philosophical works.

Philanthropy in Toni Morrison s Oeuvre

Below, I will analyze the reasons for Morrison using this primer as an opener of her novel and the breakdown of its linguistic integrity. argue that this primer functions as I epigraph for the novel ...

Author: Rico Hollmach

Publisher: Cambridge Scholars Publishing

ISBN: 9781527521049

Category: Literary Criticism

Page: 302

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This book examines Toni Morrison’s highly influential works through the lens of philanthropy. The point of departure of this endeavor is the keen observation that philanthropy has always played a leading role in US discourses about the nation itself. While doing so, time and again philanthropy has also been used as a means of social stratification – especially for so-called social minorities such as the African American community, whose historical experience within the United States is at the very heart of Morrison’s novels. This book pursues the goal of a twofold understanding – on the one hand, through offering a rather innovative access to Morrison’s works, the project allows for new insights into one of today’s most influential authors. On the other hand, this book explores the productivity of the concept of philanthropy for literary and cultural studies – a concept hitherto largely neglected by scholars in both academic fields.

The Oral Microbiome in an Ecological Perspective

The aim of this study was firstly, to design novel primers for 16S rRNA-based community profiling of the microbiota associated with carious dentine, and, secondly, to compare the results obtained with cultural and pyrosequencing ...

Author: Egija Zaura

Publisher: Frontiers Media SA

ISBN: 9782889195763

Category: Infectious and parasitic diseases

Page: 118

View: 881

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The oral cavity harbors an immense diversity of microorganisms, including bacteria, fungi, archaea, protozoa and viruses. At health, oral microbial community is thought to be in a state of homeostasis, even after numerous perturbations (e.g., toothbrushing, food intake) a day. The breach in this homeostasis can occur for instance if the perturbations become too excessive (e.g., frequent carbohydrate intake leading to acidification of the community) or the host is compromised (e.g., inadequate immune response resulting in persistent inflammation of periodontal tissue). Aggressive antimicrobial therapy (e.g., antibiotics in case of periodontal disease or preventive antibiotic therapy before and after dental extractions) is commonly applied with all the negative consequences of this approach. So far little is known on the interplay between the environmental, host and microbial factors in maintaining an ecological balance. What are the prerequisites for a healthy oral ecosystem? Can we restore an unbalanced oral microbiome? How stable is the oral microbiome through time and how robust it is to external perturbations? Gaining new insights in the ecological factors sustaining oral health will lead to conceptually new therapies and preventive programs. Recent advances in high throughput technologies have brought microbiology as a science to a new era, allowing an open-ended approach instead of focusing on few opportunistic pathogens. With this topic we would like to integrate the current high-throughput ‘omics’ tools such as metagenomics, metatranscriptomics, metaproteomics or metabolomics with biochemical, physiological, genetic or clinical parameters within the oral microbial ecosystem. We aim to address questions underlying the regulation of the ecological balance in the oral cavity by including the following areas: • Ecology of oral microbiome at health • Ecology of oral microbiome under oral diseases • Ecology of oral microbiome during non-oral diseases • Shifts in the oral microbiome by therapeutic approaches (e.g., antimicrobials, replacement therapy, pre- and probiotics) • Modeling of oral ecological shifts (e.g., animal models, in vitro microcosm models) • Complex inter- and intra-kingdom interactions (e.g., bacterial-fungal-host) related to oral ecology • Environmental (e.g., diet, tobacco), host-related (e.g., immune response, saliva composition and flow) and biotic (e.g., bacterial competition) factors influencing oral ecology • Geographic variation in oral microbial ecology and diversity

The Athenaeum

Beasley's Arithmetical Table - Book and Mental Reckoning . every day life , told in a congenial spirit . " - Spectator . ... First Books . Clarke's ( Rev. J. ) English Primer ; or , First Book in Spelling and Reading . 18mo . 6d .

Author:

Publisher:

ISBN: ONB:+Z259087407

Category:

Page: 728

View: 740

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